| Text sections: | Chapter 21
Chapter 22 Chapter 23 |
Several topics were covered. The following is a
list of study points.
| 1. | Interaction of light with matter - scattering
vs. absorption vs. emission General Spectroscopic Experiments - absorption vs. fluorescence/phosphorescence vs. other emission experiments Figures - 21-4, 21-5, 21-6 |
| 2. | Absorption Spectrometer - three parts (source, sample holder,
detector) - single vs. double beam Figures - 22-4, 22-5, 22-6, 22-11 |
| 3. | Beer's Law - A = ebc
- definitions of %T, A, etc. - finding unknown concentration of a single analyte using a single reference - finding unknown concentration of a single analyte using a calibration line - finding unknown concentration of a single analyte using a single spiking experiment - finding unknown concentration of a single analyte using a spiking experiment and calibration line - finding concentration of 2 analytes using experiments at 2 wavelengths Figures - 22-7, 22-9 |
| 4. | Fluorescence and Phosphorescence - electronic description
of the difference between absorbance, fluorescence, and phosphorescence
- relaxation pathways for electronically excited molecules - description of chemiluminescence and electroluminescence - quantitation, as in #3 |
| 5. | Infrared Absorption - electronic description, comparison with
UV-vis absorption - functional group peaks: O-H, N-H, C=O, C-O, C-H (3000 cm-1) - quantum mechanical description of vibrational energy levels - instrument and sample preparation |
| Suggested Exercises: | Chapter 21: 9, 11, 18, 26 (unknown
X has A = 0.50) Chapter 23: 13, 23, 26 |
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Last updated, January 28, 2005
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